Fig 1: High glucose induces proinflammatory polarization of macrophages. The effects of high glucose on mouse macrophage polarization were assessed by evaluating the expression levels of several critical M1/M2 macrophage markers using ELISA. (A) ELISA for IL-6 (B) ELISA for IL-1ß (C) ELISA for TNFa (D) ELISA for IFN? (E) ELISA for Arg1 (F) ELISA for CD163. *p<0.05.
Fig 2: Res promoted the polarization of microglia to M2 type and play an anti-inflammatory role. (A) Protein levels of Arg-1 in microglia in the hippocampal CA1 region; (B) protein levels of iNOS in microglia in the hippocampal CA1 region; (C) percentage of iNOS+DAPI+/Iba-1+DAPI+ and Arg-1+DAPI+/Iba-1+DAPI+; (D) percentage of Iba-1+DAPI+/DAPI+; (E) fluorescent labeling of M1 and M2 microglia; (F) the levels of IL-1ß, IL-4, IL-10 and TNF-a in hippocampus (pg/mL) in different groups were detected. *P<0.05 indicated vs Con group; #P<0.05 indicated vs PTX group; &P<0.05 indicated vs PTX+Res group; $P<0.05 indicated vs PTX group; ns indicated no statistical significance. Scale bar = 50 µm.
Fig 3: NLRP3 depletion in microglia reduces phagocytosis potential and release of pro-inflammatory cytokines. (A) Phagocytosis for zymosan was assessed in sorted disc microglia from mice with microglia-specific alteration in NLRP3 expression. (B) ELISA for IL-1ß, TNFa, IFN?, ARG1 and CD163 in sorted disc microglia from mice with microglia-specific alteration in NLRP3 expression. The relative levels to those from NLRP3mut (=1) were shown. *p<0.05. ns, non-significant.
Supplier Page from Abcam for Mouse Arginase 1 ELISA Kit